3NT9
CRYSTAL STRUCTURE OF LSSmKate1 red fluorescent proteins with large Stokes shift
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 43 |
| Unit cell lengths | 72.177, 72.177, 226.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.990 |
| R-factor | 0.18463 |
| Rwork | 0.182 |
| R-free | 0.23179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.835 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
| High resolution limit [Å] | 1.990 | 5.400 | 1.990 |
| Rmerge | 0.075 | 0.034 | 0.717 |
| Number of reflections | 76485 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 96.7 | 99.5 | 66.8 |
| Redundancy | 6.7 | 7.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 20% PEG 1000, 0.1 M phosphate-citrate, pH 5.6, 0.2 M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
