3NT3
CRYSTAL STRUCTURE OF LSSmKate2 red fluorescent proteins with large Stokes shift
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.704, 49.454, 127.171 |
| Unit cell angles | 90.00, 93.13, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.500 |
| R-factor | 0.1753 |
| Rwork | 0.174 |
| R-free | 0.20180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kcs |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.672 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.052 | 0.040 | 0.398 |
| Number of reflections | 137175 | ||
| <I/σ(I)> | 14.6 | ||
| Completeness [%] | 96.2 | 97.1 | 94 |
| Redundancy | 4.9 | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.1 M Na-acetate, 25% PEG 3350, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
