3NSY
The multi-copper oxidase CueO with six Met to Ser mutations (M358S,M361S,M362S,M364S,M366S,M368S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.3799 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.052, 91.259, 53.979 |
| Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.100 |
| R-factor | 0.17253 |
| Rwork | 0.170 |
| R-free | 0.22726 |
| Structure solution method | UNnecessary |
| Starting model (for MR) | 1kv7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.315 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.043 | 0.130 |
| Number of reflections | 47535 | |
| <I/σ(I)> | 11.2 | 4.7 |
| Completeness [%] | 97.0 | 95 |
| Redundancy | 1.9 | 1.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 16% PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
