3NSX
The crystal structure of the The crystal structure of the D420A mutant of the alpha-glucosidase (FAMILY 31) from Ruminococcus obeum ATCC 29174
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.827, 120.021, 88.247 |
| Unit cell angles | 90.00, 109.10, 90.00 |
Refinement procedure
| Resolution | 39.735 - 1.569 |
| R-factor | 0.1548 |
| Rwork | 0.153 |
| R-free | 0.17870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n04 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.600 |
| High resolution limit [Å] | 1.569 | 1.570 |
| Rmerge | 0.063 | 0.483 |
| Number of reflections | 193344 | |
| <I/σ(I)> | 29.6 | 2.3 |
| Completeness [%] | 99.7 | 96 |
| Redundancy | 4.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1M BIS-TRIS, 25% PEG3350, 10% isomaltose, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
