3NSM
Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 from Ostrinia furnacalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 108.110, 108.110, 175.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.610 - 2.100 |
R-factor | 0.21738 |
Rwork | 0.217 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o7a |
RMSD bond length | 0.007 |
RMSD bond angle | 1.095 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.630 |
High resolution limit [Å] | 2.100 |
Number of reflections | 58734 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100mM HEPES, 200mM MgCl2, 30% PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |