3NSI
Crystal Structure of the Post-Refolded S100A3 Protein Expressed in Insect Cell
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-10-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 58.680, 58.680, 45.763 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.20501 |
| Rwork | 0.203 |
| R-free | 0.23645 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nsk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.607 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 9598 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Ammonium Sulfate, 0.1M Bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
