3NSD
Silver bound to the multicopper oxidase CueO (untagged)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.181, 90.324, 53.810 |
| Unit cell angles | 90.00, 102.26, 90.00 |
Refinement procedure
| Resolution | 26.400 - 2.000 |
| R-factor | 0.18965 |
| Rwork | 0.187 |
| R-free | 0.23782 |
| Structure solution method | UNnecessary |
| Starting model (for MR) | 1kv7 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.655 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.400 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.330 |
| Number of reflections | 30337 | |
| <I/σ(I)> | 7.5 | 2.1 |
| Completeness [%] | 96.0 | 93 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 16% polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6; , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
