3NS6
Crystal structure of hte RNA recognition motif of yeast eIF3b residues 76-170
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.713, 51.752, 77.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.026 - 1.250 |
R-factor | 0.1429 |
Rwork | 0.142 |
R-free | 0.16750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sxl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.326 |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.026 | 1.330 | |
High resolution limit [Å] | 1.250 | 30.000 | 1.250 |
Rmerge | 0.081 | 0.038 | 0.503 |
Number of reflections | 48971 | 5 | 8169 |
<I/σ(I)> | 12.42 | 19.5 | 4.2 |
Completeness [%] | 99.6 | 55.6 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 30% PEG4000, 200 mM Li2SO4 and 100 mM Hepes pH 8. The crystals were grown in a sitting drop at 20 degrees by mixing 1uL of protein (11 mg/mL) with 1uL of reservoir, VAPOR DIFFUSION, SITTING DROP |