3NS6

Crystal structure of hte RNA recognition motif of yeast eIF3b residues 76-170

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	43.713, 51.752, 77.095
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.026 - 1.250
R-factor	0.1429
Rwork	0.142
R-free	0.16750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sxl
RMSD bond length	0.010
RMSD bond angle	1.326
Data scaling software	XSCALE
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.026		1.330
High resolution limit [Å]	1.250	30.000	1.250
Rmerge	0.081	0.038	0.503
Number of reflections	48971	5	8169
<I/σ(I)>	12.42	19.5	4.2
Completeness [%]	99.6	55.6	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8		30% PEG4000, 200 mM Li2SO4 and 100 mM Hepes pH 8. The crystals were grown in a sitting drop at 20 degrees by mixing 1uL of protein (11 mg/mL) with 1uL of reservoir, VAPOR DIFFUSION, SITTING DROP