3NS5
Crystal structure of the RNA recognition motif of yeast eIF3b residues 76-161
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Wavelength(s) | 1.5417 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.280, 68.140, 81.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.456 - 2.598 |
R-factor | 0.2274 |
Rwork | 0.225 |
R-free | 0.27250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ns6 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.480 |
Data scaling software | SCALA |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.160 | 34.070 | 2.730 |
High resolution limit [Å] | 2.598 | 8.200 | 2.590 |
Rmerge | 0.031 | 0.320 | |
Total number of observations | 795 | 3276 | |
Number of reflections | 5632 | ||
<I/σ(I)> | 10.8 | 19.7 | 2.4 |
Completeness [%] | 99.1 | 98.8 | 94.6 |
Redundancy | 4.4 | 3.7 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 33% PEG4000 and 0.1 M Na-citrate (pH 5.6) in a sitting drop plate at 20 degrees. 1uL of protein at the concentration of 17 mg/mL was mixed with 1 uL of reservoir, VAPOR DIFFUSION, SITTING DROP |