3NS4
Structure of a C-terminal fragment of its Vps53 subunit suggests similarity of GARP to a family of tethering complexes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2009-10-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 127.236, 127.236, 81.658 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.400 - 2.900 |
R-factor | 0.19114 |
Rwork | 0.190 |
R-free | 0.22072 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.768 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 8606 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 50 mM Tris at pH 8.5, 10% PEG 4K, 200 mM BaCl2, and 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |