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3NRX

Insights into anti-parallel microtubule crosslinking by PRC1, a conserved non-motor microtubule binding protein

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-E
Synchrotron siteAPS
Beamline24-ID-E
Temperature [K]173
Detector technologyCCD
Collection date2009-04-21
DetectorADSC QUANTUM 315
Spacegroup nameP 32
Unit cell lengths49.078, 49.078, 94.784
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution25.000 - 1.750
R-factor0.202
Rwork0.198
R-free0.26900
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3nry
RMSD bond length0.008
RMSD bond angle1.030
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareREFMAC (5.5.0102)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.0001.810
High resolution limit [Å]1.7501.750
Rmerge0.0530.361
Number of reflections24182
<I/σ(I)>31.42.62
Completeness [%]94.094.8
Redundancy4.34.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
19.52771:1 v/v of protein ( 50 mg/ml in 80 mM PIPES, pH6.8, 1 mM MgCl2, 1 mM EDTA and 150 mM KCl) and reservoir buffer (100 mM CHES, pH 9.5, 30% PEG3000), VAPOR DIFFUSION, HANGING DROP, temperature 277K

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