3NRV
Crystal structure of MarR/EmrR family transcriptional regulator from Acinetobacter sp. ADP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-26 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.809, 59.573, 97.753 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 32.584 - 2.001 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.22950 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.514 |
| Number of reflections | 53697 | |
| <I/σ(I)> | 39 | 4 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M bis-tris pH 6.5; 2M NH4SO4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
