3NRU
Ligand binding domain of EPHA7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 137.617, 140.699, 143.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.300 |
| R-factor | 0.2368 |
| Rwork | 0.235 |
| R-free | 0.26894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wo1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.967 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 123900 | |
| <I/σ(I)> | 36.4 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.3 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 291 | 0.3 M NH4SO4, 0.6 M LiSO4, 0.1 M tri-NaCitrate pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
