3NR8
Crystal structure of human SHIP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-22 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.796, 61.177, 114.320 |
Unit cell angles | 90.00, 91.90, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.21644 |
Rwork | 0.214 |
R-free | 0.27247 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i9y |
RMSD bond length | 0.009 |
RMSD bond angle | 1.146 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.770 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.152 | 0.720 |
Number of reflections | 15338 | |
<I/σ(I)> | 7.9 | 2 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 20% PEG 6000, 0.1M citric acid pH 5, 2mM biphenyl 2,3',4,5',6-pentakisphosphate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |