3NR7
Crystal structure of S. typhimurium H-NS 1-83
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-17 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 130.397, 130.397, 55.061 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.600 - 3.700 |
R-factor | 0.27366 |
Rwork | 0.271 |
R-free | 0.30375 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ov9 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.551 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.600 | 3.830 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.100 | 0.440 |
Number of reflections | 5676 | |
<I/σ(I)> | 6.7 | 1.8 |
Completeness [%] | 96.9 | 96.4 |
Redundancy | 2.7 | 2.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.2 | 290 | 20 % (v/v) MPD, 20 mM phenol, 100 mM MES, pH 6.2, MICRODIALYSIS, temperature 290K |