3NR1
A metazoan ortholog of SpoT hydrolyzes ppGpp and plays a role in starvation responses
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2007-09-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97953 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.900, 62.400, 53.900 |
| Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.203 |
| R-free | 0.23020 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.077 | 0.051 | 0.354 |
| Number of reflections | 28330 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 99.8 | 99.2 | 99.5 |
| Redundancy | 3.7 | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 20-25% PEG 3350, 0.2M sodium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 20-25% PEG 3350, 0.2M sodium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
