3NQX

Crystal structure of vibriolysin MCP-02 mature enzyme, a zinc metalloprotease from M4 family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-12-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.48
Spacegroup name	P 21 21 21
Unit cell lengths	43.801, 73.792, 91.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.450 - 1.700
R-factor	0.17649
Rwork	0.174
R-free	0.21739
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ezm
RMSD bond length	0.015
RMSD bond angle	1.608
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.450	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.185	0.180
Number of reflections	32935
<I/σ(I)>	54	9
Completeness [%]	100.0	100
Redundancy	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	12% PEG 3000, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K