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3NPH

Crystal structure of the pfam00427 domain from Synechocystis sp. PCC 6803

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU FR-E+ DW
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2009-11-07
DetectorRIGAKU RAXIS IV++
Wavelength(s)1.498
Spacegroup nameP 41 21 2
Unit cell lengths55.675, 55.675, 79.187
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution22.772 - 1.849
R-factor0.204
Rwork0.203
R-free0.23430
Structure solution methodSAD
RMSD bond length0.006
RMSD bond angle0.988
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareSOLVE
Refinement softwarePHENIX ((phenix.refine))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]39.4301.910
High resolution limit [Å]1.8491.849
Number of reflections11164
Completeness [%]100.0100
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.529812% PEG 3000, 0.1M Bis-Tris buffer (pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K

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