3NPH
Crystal structure of the pfam00427 domain from Synechocystis sp. PCC 6803
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-11-07 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.498 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.675, 55.675, 79.187 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.772 - 1.849 |
R-factor | 0.204 |
Rwork | 0.203 |
R-free | 0.23430 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.988 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.430 | 1.910 |
High resolution limit [Å] | 1.849 | 1.849 |
Number of reflections | 11164 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12% PEG 3000, 0.1M Bis-Tris buffer (pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K |