3NOY
Crystal structure of IspG (gcpE)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.7362, 1.7421, 1.7122 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 243.700, 119.010, 63.820 |
| Unit cell angles | 90.00, 95.48, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.22375 |
| Rwork | 0.221 |
| R-free | 0.26614 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.002 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.700 |
| Rmerge | 0.054 | 0.419 |
| Number of reflections | 55518 | |
| <I/σ(I)> | 19 | 3.5 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100mM Bis-Tris, 9% PEG 8000, 17mM SrCl2, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
