3NOW
UNC-45 from Drosophila melanogaster
Experimental procedure
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 2 3 |
Unit cell lengths | 184.079, 184.079, 184.079 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.197 - 2.992 |
R-factor | 0.2024 |
Rwork | 0.201 |
R-free | 0.23550 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.293 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.5_2) |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 2.992 | 2.992 |
Number of reflections | 42099 | |
<I/σ(I)> | 19.2 | |
Completeness [%] | 99.8 | 97.8 |
Redundancy | 9.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1 M sodium citrate, 0.2 M ammonium acetate, 25% (w/v) PEG 4000, 1% (v/v) ethylene glycol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |