3NOP
Light-induced intermediate structure L1 of Pseudomonas aeruginosa bacteriophytochrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 154.380, 162.979, 436.098 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| Structure solution method | difference Fourier Method |
| Starting model (for MR) | 3nhq |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | DynamiX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.118 |
| Number of reflections | 131216 |
| Completeness [%] | 82.8 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.7 | 293 | 10mg/ml protein, 0.1M Tris HCl buffer, 0.45M ammonium phosphate, pH 7.7, VAPOR DIFFUSION, temperature 293K |
