3NOK
Crystal structure of Myxococcus xanthus Glutaminyl Cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.790, 46.060, 81.000 |
| Unit cell angles | 90.00, 112.97, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.650 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.23610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nom |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.072 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.750 | |
| High resolution limit [Å] | 1.650 | 30.000 | 1.650 |
| Rmerge | 0.059 | 0.546 | |
| Rmeas | 0.652 | ||
| Number of reflections | 52886 | 8165 | |
| <I/σ(I)> | 15.56 | 2.3 | |
| Completeness [%] | 97.9 | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
