3NOE
Crystal Structure of Dihydrodipicolinate synthase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 292 |
Detector technology | IMAGE PLATE |
Collection date | 2009-09-28 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.541 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.420, 81.870, 124.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.910 - 2.950 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yxc |
RMSD bond length | 0.008 |
RMSD bond angle | 1.700 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.910 | 3.100 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 12763 | |
Completeness [%] | 97.0 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG 3350, 120mM MgCl2, 70mM Tris-HCl, 6% propylene glycol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |