3NO8
Crystal structure of the PHR domain from human BTBD2 Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.513, 59.513, 175.919 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.930 - 2.200 |
| R-factor | 0.2021 |
| Rwork | 0.199 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Poly-ala model of PDB Code: 3GBW |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.622 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.070 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 16797 | |
| <I/σ(I)> | 15 | 6.9 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 26.4 | 27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 294 | 100mM citric acid, 2000mM ammonium sulfate, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
