3NO7
Crystal structure of the centromere-binding protein ParB from plasmid pCXC100
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.186, 74.186, 52.596 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.19818 |
| Rwork | 0.197 |
| R-free | 0.21243 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.113 | 0.627 |
| Number of reflections | 33276 | |
| <I/σ(I)> | 28 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.8M Li2SO4, 0.01M MgSO4, 0.05M cacodylate-Na, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
