3NNK
Biochemical and Structural Characterization of a Ureidoglycine Aminotransferase in the Klebsiella pneumoniae Uric Acid Catabolic Pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 142.150, 149.203, 197.996 |
| Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.580 |
| R-factor | 0.21882 |
| Rwork | 0.217 |
| R-free | 0.24602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vj0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.992 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Number of reflections | 240000 | |
| <I/σ(I)> | 8.6 | 1.9 |
| Completeness [%] | 96.8 | 96.8 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 34% MPD, 0.1 M NaCl, 0.1 M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K, temperature 291K |
