3NNH
Crystal Structure of the CUGBP1 RRM1 with GUUGUUUUGUUU RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.180, 62.074, 122.052 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.596 - 2.750 |
R-factor | 0.1909 |
Rwork | 0.183 |
R-free | 0.26280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nmr |
RMSD bond length | 0.007 |
RMSD bond angle | 1.031 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.114 | 0.376 |
Number of reflections | 12357 | |
<I/σ(I)> | 13.1 | 4.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.3 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M potassium sodium tartrate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |