3NMV
Crystal structure of pyrabactin-bound abscisic acid receptor PYL2 mutant A93F in complex with type 2C protein phosphatase ABI2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.129, 97.587, 134.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.100 |
| R-factor | 0.19553 |
| Rwork | 0.193 |
| R-free | 0.22281 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nmt |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.950 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.100 |
| Number of reflections | 44745 |
| <I/σ(I)> | 32.4 |
| Completeness [%] | 98.2 |
| Redundancy | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 10% PEG 8000, 10% sucrose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
