3NMR
Crystal Structure of CUGBP1 RRM1/2-RNA Complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930, 0.97890, 0.96360 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 47.548, 70.012, 132.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.20562 |
| Rwork | 0.204 |
| R-free | 0.24451 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.257 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.082 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.369 | |
| Number of reflections | 18231 | |
| <I/σ(I)> | 36.7 | 3.6 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2 M ammonium acetate, 45% MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
