3NMH
Crystal structure of the abscisic receptor PYL2 in complex with pyrabactin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.268, 105.074, 185.076 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.510 - 1.850 |
R-factor | 0.19736 |
Rwork | 0.194 |
R-free | 0.23479 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kb0 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.493 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.850 |
Rmerge | 0.100 |
Number of reflections | 51071 |
<I/σ(I)> | 32.2 |
Completeness [%] | 93.6 |
Redundancy | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2M ammonium sulfate, 0.1M HEPES, 10 % glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |