3NME
Structure of a plant phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-09 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.408, 73.943, 162.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.400 |
| R-factor | 0.20234 |
| Rwork | 0.199 |
| R-free | 0.24959 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.447 |
| Number of reflections | 26660 | |
| <I/σ(I)> | 11.4 | 2.04 |
| Completeness [%] | 99.2 | 94.7 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.4 M lithium citrate, 24% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
