3NMD

Crystal structure of the leucine zipper domain of cGMP dependent protein kinase I beta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	90
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	62.774, 77.312, 148.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.272
R-factor	0.1973
Rwork	0.195
R-free	0.24750
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.779
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.000
High resolution limit [Å]	2.270
Number of reflections	32085
<I/σ(I)>	23.6
Completeness [%]	99.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	278	0.1M sodium citrate (pH 5.6) and 2.5 mM hexanediol, VAPOR DIFFUSION, SITTING DROP, temperature 278K