3NKZ
The crystal structure of a flagella protein from Yersinia enterocolitica subsp. enterocolitica 8081
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.798, 66.758, 137.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.936 - 2.112 |
| R-factor | 0.1582 |
| Rwork | 0.159 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.051 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.160 |
| High resolution limit [Å] | 2.112 | 2.120 |
| Rmerge | 0.093 | 0.754 |
| Number of reflections | 34714 | |
| <I/σ(I)> | 31.9 | 2.44 |
| Completeness [%] | 96.2 | 96.5 |
| Redundancy | 6.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1.0M Ammonium sulfate, 0.1M Bis-Tris, 1% w/v PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
