3NK6
Structure of the Nosiheptide-resistance methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0055 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.884, 69.203, 64.797 |
| Unit cell angles | 90.00, 117.85, 90.00 |
Refinement procedure
| Resolution | 33.469 - 2.000 |
| R-factor | 0.2039 |
| Rwork | 0.201 |
| R-free | 0.25370 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.228 | |
| Number of reflections | 33406 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 97.4 | 95.2 |
| Redundancy | 7.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 0.35M ammonium chloride, 24%(w/v) PEG 3350, 0.1M MES, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
