3NK4
Crystal structure of full-length sperm receptor ZP3 at 2.0 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9757 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 97.700, 97.700, 256.419 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.344 - 2.000 |
R-factor | 0.2092 |
Rwork | 0.208 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3D4G: PDB ENTRY 3D4G: RESIDUES 372-449 + 459-473 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.224 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.900 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.092 | |
Number of reflections | 84686 | |
<I/σ(I)> | 12.6 | 2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.3 | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 25 MG/ML PROTEIN IN 0.1 M SODIUM CITRATE, 0.01 M TRIS-HCL, 5% PEG6000, 0.05 M SODIUM CHLORIDE, SAMPLE TO RESERVOIR RATIO IN DROP: 1:1 (DROP), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |