3NJX
Rhamnogalacturonan Lyase from Aspergillus aculeatus mutant H210A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-30 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.071, 77.071, 171.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.940 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nkg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.993 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.020 |
| High resolution limit [Å] | 1.940 | 1.950 |
| Rmerge | 0.113 | 0.305 |
| Number of reflections | 35438 | |
| <I/σ(I)> | 17.2 | 6.8 |
| Completeness [%] | 91.3 | 90.6 |
| Redundancy | 7.5 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 20% PEG4000, 0.1 M Ammonium Sulphate with 9% PEG400 as cryo protectant, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
