3NJL
D116A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis, at pH7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-13 |
Detector | SBC-3 |
Wavelength(s) | 0.9792 |
Spacegroup name | H 3 2 |
Unit cell lengths | 100.151, 100.151, 100.216 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.200 - 1.750 |
R-factor | 0.166 |
Rwork | 0.166 |
R-free | 0.17200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n55 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.602 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.200 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.043 | 0.786 |
Number of reflections | 19469 | |
<I/σ(I)> | 11.1 | 2.45 |
Completeness [%] | 99.6 | 99.1 |
Redundancy | 10.3 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M HEPES buffer, 0.5 M magnesium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |