3NJC
Crystal structure of the yslB protein from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR460.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97890 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.827, 52.105, 136.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.207 - 1.693 |
| R-factor | 0.1875 |
| Rwork | 0.185 |
| R-free | 0.22840 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 500.000 | 500.000 | 1.760 |
| High resolution limit [Å] | 1.693 | 3.660 | 1.700 |
| Rmerge | 0.060 | 0.045 | 0.369 |
| Rmeas | 0.060 | ||
| Total number of observations | 495994 | ||
| Number of reflections | 71912 | ||
| <I/σ(I)> | 15.7 | ||
| Completeness [%] | 94.1 | 90.8 | 91.5 |
| Redundancy | 6.9 | 9.6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 18-20% PEG 4000, 0.1M HEPES, pH7.0, hanging drop at 291K, VAPOR DIFFUSION, HANGING DROP |
