3NJ6
0.95 A resolution X-ray structure of (GGCAGCAGCC)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 46.427, 46.427, 82.678 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.210 - 0.950 |
| R-factor | 0.106 |
| Rwork | 0.106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A-RNA HELIX |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.996 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.1) |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.213 | 0.970 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Number of reflections | 21756 | |
| <I/σ(I)> | 74.1 | 7.7 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 15.3 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 292 | MgSO4, (NH4)2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
