3NJ2
Crystal structure of cce_0566 from the cyanobacterium Cyanothece 51142, a protein associated with nitrogen fixation from the DUF269 family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.079, 44.573, 67.724 |
| Unit cell angles | 90.00, 111.01, 90.00 |
Refinement procedure
| Resolution | 33.000 - 1.590 |
| R-factor | 0.2123 |
| Rwork | 0.210 |
| R-free | 0.25452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g7p |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 1.634 |
| High resolution limit [Å] | 1.590 | 1.593 |
| Number of reflections | 38536 | |
| Completeness [%] | 91.7 | 75.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 0.2 M Ammonium phosphate monobasic, 0.1 M Tris pH 8.5, 50% v/v (+/-)-2-Methyl-2,4-pentanediol, protein in 100 mM NaCl, 20 mM Tris, 1 mM DTT, pH 7.1. Protein 2 mg/mL, Mix 1.5 uL of protein and precipatant, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
