3NIZ

Cryptosporidium parvum cyclin-dependent kinase cgd5_2510 with ADP bound.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-11-28
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97934
Spacegroup name	P 43 21 2
Unit cell lengths	64.337, 64.337, 200.039
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.400
R-factor	0.2225
Rwork	0.221
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.060	0.038	0.372
Number of reflections	24975
<I/σ(I)>	10.3		2.7
Completeness [%]	97.5	99.3	80.8
Redundancy	12.4	12.9	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	18% PEG 3350 0.15 M MgAcet 15 % ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K