3NIZ
Cryptosporidium parvum cyclin-dependent kinase cgd5_2510 with ADP bound.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-11-28 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97934 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 64.337, 64.337, 200.039 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.400 |
R-factor | 0.2225 |
Rwork | 0.221 |
R-free | 0.25640 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.060 | 0.038 | 0.372 |
Number of reflections | 24975 | ||
<I/σ(I)> | 10.3 | 2.7 | |
Completeness [%] | 97.5 | 99.3 | 80.8 |
Redundancy | 12.4 | 12.9 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 18% PEG 3350 0.15 M MgAcet 15 % ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |