3NIY
Crystal structure of native xylanase 10B from Thermotoga petrophila RKU-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.458 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.538, 58.554, 61.579 |
| Unit cell angles | 84.54, 70.82, 68.87 |
Refinement procedure
| Resolution | 31.240 - 1.580 |
| R-factor | 0.1598 |
| Rwork | 0.158 |
| R-free | 0.19551 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.415 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.240 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.101 | |
| Number of reflections | 91930 | |
| Completeness [%] | 92.8 | 86.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 291 | PEG8000 glycerol sodium acetate litium sulfate, pH 4.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
