3NI8
Crystal Structure of PFC0360w, an HSP90 activator from plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-20 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.658, 96.658, 44.288 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.700 - 2.500 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.392 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.700 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.159 | 0.836 |
| Number of reflections | 8517 | |
| <I/σ(I)> | 2.8 | 1.42 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 10.6 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20% isopropanol, 20% PEG 4000, 0.1M Na Citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
