3NHZ
Structure of N-terminal Domain of MtrA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-23 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 31 |
| Unit cell lengths | 56.600, 56.600, 181.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.340 - 2.500 |
| Rwork | 0.205 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gwr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.293 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (AUTOMR) |
| Refinement software | PHENIX (refine) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.102 | 0.540 |
| Number of reflections | 25644 | |
| <I/σ(I)> | 9.1 | 3.18 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 2.6 M NaCl, 100 mM HEPES pH 7.5 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
