3NHQ
The dark Pfr structure of the photosensory core module of P. aeruginosa Bacteriophytochrome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-19 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97857 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 154.380, 162.979, 436.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.480 - 2.550 |
R-factor | 0.2174 |
Rwork | 0.215 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c2w |
RMSD bond length | 0.004 |
RMSD bond angle | 0.868 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.069 | 0.404 |
Number of reflections | 148709 | |
<I/σ(I)> | 23 | 1.7 |
Completeness [%] | 79.0 | 29.4 |
Redundancy | 5.5 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.7 | 293 | 10mg/ml protein, 0.45 M ammonium phosphate, 0.1 M Tris-HCl, pH 7.7, VAPOR DIFFUSION, temperature 293K |