3NHM
Crystal structure of a response regulator from Myxococcus xanthus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.616, 40.408, 99.617 |
| Unit cell angles | 90.00, 102.28, 90.00 |
Refinement procedure
| Resolution | 48.670 - 2.190 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gt7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.670 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.087 | 0.310 |
| Number of reflections | 11270 | |
| <I/σ(I)> | 9.8 | 2.5 |
| Completeness [%] | 95.9 | 84.3 |
| Redundancy | 7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M Potassium Thiocyanate, 30% Polyethylene Glycol Monomethyl Ether 2000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
