3NH9
Nucleotide Binding Domain of Human ABCB6 (ATP bound structure)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-29 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.152, 65.626, 69.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.520 - 2.100 |
R-factor | 0.179 |
Rwork | 0.175 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nh6 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.744 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.520 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | |
Number of reflections | 15449 | 1021 |
<I/σ(I)> | 15.8 | 2.9 |
Completeness [%] | 97.7 | 90.4 |
Redundancy | 3.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES pH 6.5, 40% (v/v) PEG400, 30mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 298K |