3NH7
Crystal structure of the neutralizing Fab fragment AbD1556 bound to the BMP type I receptor IA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.320, 129.255, 100.239 |
| Unit cell angles | 90.00, 92.27, 90.00 |
Refinement procedure
| Resolution | 30.400 - 2.700 |
| R-factor | 0.23643 |
| Rwork | 0.234 |
| R-free | 0.28044 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aqk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.624 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.341 |
| Number of reflections | 59539 | |
| <I/σ(I)> | 8.2 | 2.7 |
| Completeness [%] | 95.3 | 97.8 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 100mM Tris-HCl pH 7.0, 20% (w/v) PEG 8000 and 10% (w/v) glucose , VAPOR DIFFUSION, HANGING DROP, temperature 294K |
