3NGQ
Crystal structure of the human CNOT6L nuclease domain
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9790 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 77.152, 77.152, 165.897 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.22129 |
Rwork | 0.220 |
R-free | 0.25413 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.793 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.800 | 1.940 |
Rmerge | 0.076 | 0.463 |
Number of reflections | 45797 | |
<I/σ(I)> | 47.7 | 2.9 |
Completeness [%] | 85.1 | 75.2 |
Redundancy | 12.3 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M Hepes, pH 7.5, 1.1M ammonium tartrate, 0.2M NDSB-201, VAPOR DIFFUSION, HANGING DROP, temperature 291K |