3NGI
RB69 DNA Polymerase (Y567A) Ternary Complex with dTTP Opposite dG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.989, 120.336, 130.768 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.886 |
R-factor | 0.19371 |
Rwork | 0.192 |
R-free | 0.22623 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nci |
RMSD bond length | 0.007 |
RMSD bond angle | 1.010 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.110 | 1.000 |
Number of reflections | 95329 | |
<I/σ(I)> | 12.4 | 1.04 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | micro-batch vapor-diffusion | 6.5 | 293 | 13%(w/v) PEG 350 monomethyl ether (MME), 150 mM CaCl2, 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K |